Genlantis SoluBL21 說(shuō)明書(shū)

世界*實(shí)驗(yàn)材料供應(yīng)商 Genlantis Inc. 正式授權(quán)上海起發(fā)為其中國(guó)代理， Genlantis Inc. 在一直是行業(yè)的*,一直為廣大科研客戶(hù)提供zui為的產(chǎn)品和服務(wù),上海起發(fā)一直秉承為中國(guó)科研客戶(hù)帶來(lái)的產(chǎn)品，的服務(wù)，簽約 Genlantis Inc. 就是為了給廣大科研客戶(hù)帶來(lái)更加完善的產(chǎn)品和服務(wù),您的滿(mǎn)意將是我們zui大的收獲

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Genlantis位于美國(guó)加利福尼亞州的圣地亞哥，是基因治療系統(tǒng)公司的分公司。Genlantis致力于體內(nèi)和體外診斷蛋白表達(dá)工具的研發(fā)及傳遞技術(shù)的開(kāi)發(fā)。Genlantis為的生命科學(xué)研究客戶(hù)生產(chǎn)研發(fā)生物學(xué)試劑，包括基因傳遞和質(zhì)粒DNA的轉(zhuǎn)染試劑（GenePORTER®），哺乳動(dòng)物細(xì)胞siRNA轉(zhuǎn)染試劑（GeneSilencer®），原代神經(jīng)元細(xì)胞的優(yōu)化轉(zhuǎn)染試劑（NeuroFECT®）和原代神經(jīng)細(xì)胞的轉(zhuǎn)染試劑（NeuroPure®）。

此外，我們還是Tap Express®和Xi-Clone® Instant Enzymeless PCR克隆技術(shù)的持有者。這些技術(shù)使得科學(xué)家們可以同時(shí)設(shè)計(jì)成百上千的蛋白，還可把成本控制在理想的范圍內(nèi)；可以使科學(xué)家們由復(fù)雜的細(xì)菌感染比如瘧疾，肺結(jié)核，炭疽，天花病毒，衣原體，螺桿菌等快速的制備出相應(yīng)的蛋白。

• Express toxic proteins
• Optimized E. coli strain for expressing insoluble proteins in soluble form
• Simple and straightforward protocol
• Compatible with all T7 promoter-based expression vectors
• 2 White Papers (See tab above)

Genlantis is pleased to offer SoluBL21™ Competent E. coli**, a significantly improved BL21 host strain for toxic and soluble protein expression. Click on the DATA tab. Using a directed evolution approach, Genlantis scientists have developed a mutant strain of BL21 DE3 E. coli that can produce soluble protein in the majority of cases where expression in the parent BL21(DE3) yielded no detectable soluble product.

Contents

Shipping

The SoluBL21 Chemically Competent E. coli are shipped frozen. 
For maximum stability and long-term use, store the cells and pUC19 control plasmid at -70篊 upon receipt. 
All components are guaranteed stable for 6 months when stored properly

** Patent Pending

Soluble Protein Data

Figure 1: (click to enlarge) Expression of Insoluble Proteins in SoluBL21 vs. standard BL21(DE3) E. coli

Figure 1 legend: A panel of 22 clones that produced little or no soluble protein in standard BL21(DE3) cells were induced side-by-side in BL21(DE3) and SoluBL-21 E. coli. These clones encoded for a wide range of proteins including: cell cycle proteins, ATP-binding proteins, transferases, isomerases, kinases, and repair enzymes from human and other mammalian sources. With the SoluBL21 strain, ~70% of previously insoluble proteins were expressed in soluble form. Examples of SoluBL21-expressed proteins are shown above.

With Genlantis' SoluBL21 strain, a major obstacle to effective protein expression in E. coli has been overcome for many proteins. This significant improvement should enable scientists to make progress in a wide range of applications quicker and far less expensively than in the past.

Toxic Protein Data

We have evaluated the SoluBL21 host to determine whether clones toxic in BL21(DE3) and BL21(DE3)pLysS would exhibit similar toxicity in this slower growing variant. We demonstrate here that in addition to improving the solubility profile of many recombinant proteins, the SoluBL21 strain also permits the establishment of many clones that are toxic to, and cannot be established in, BL21(DE3) and BL21(DE3)pLysS. Furthermore, we show that these toxic clones produce substantial quantities of soluble protein when induced. White Paper Link

Transformation of Plasmid DNAs (Toxic Clones) into standard BL21(DE3), BL21(DE3)pLysS and SoluBL21 E. coli

Soluble Protein Fractions of "Toxic" Clones in SoluBL21 (DE3) E. coli