pEF1/myc-His C載體說(shuō)明書

產(chǎn)品參數(shù): 

Key Function：Constitutive Expression
Mammalian Selection：Neo，G418
Promoter：EF-1α
Vector Type：pEF
Cloning Method：Restriction Enzyme/MCS 
Protein Tag or Fusion：c-Myc Epitope Tag，His Tag （6x His） 
Constitutive or Inducible System：Constitutive

載體抗性: 

氨芐青霉素（Ampicillin）

載體描述: 

pEF1/myc-His A, B, and C are 6.2 kb vectors derived from pcDNA 3.1/myc-His and designed for overproduction of recombinant proteins in mammalian cell lines. Features of the vectors allow purification and detection of expressed proteins. High-level stable and transient expression can be carried out in most mammalian cells. The vectors contain the following elements:

1. Human elongation factor 1?-subunit （hEF-1α） promoter for high-level expression across a broad range of species and cell types （Goldman et al., 1996; Mizushima and Nagata, 1990）.
2. Three reading frames to facilitate in-frame cloning with a C-terminal tag encoding the myc （c-myc） epitope and a polyhistidine metal-binding peptide.
3. Neomycin resistance gene for selection of stable cell lines.
4. Episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen （e.g., COS7）.

The control plasmid, pEF1/myc-His/lacZ, is included for use as a positive control for transfection, expression, and detection in the cell line of choice.

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